| Molecular Formula | C23H32N6O14 |
| Molar Mass | 616.53 |
| Physical and Chemical Properties | Chemical properties of polyantimycin in China is colorless needle crystal, m.p.180 ℃, pure Japanese polyantimycin B is amorphous crystal, m.p.160 ℃ (decomposition). Polyanamycin is easily soluble in water, but insoluble in organic solvents such as methanol, ethanol, acetone, chloroform, benzene and ether. Stable to ultraviolet light, acidic solution, neutral solution, unstable to alkaline solution, stable at room temperature for more than 3 years. The appearance of industrial products is light brown yellow powder or grayish brown powder. |
| Use | Uses for wheat white powder disease, tobacco brown spot disease, cucumber downy mildew, melon Fusarium wilt, Ginseng leaf spot, beet brown spot disease, rice sheath blight, Apple early defoliation disease, tree blight disease, pear black spot and other fungal diseases have good control effect. Not only can cure the disease, but also can stimulate plant growth. For example, to control rice sheath blight, the control effect can reach 80%, the growth of rice is strong, the seed setting rate increased by about 6% compared with the control, the average yield increased by about 10%. |
| Raw Materials | D(+)-Glucose SOYBEAN OIL Agar D-(+)-Maltose monohydrate Maltose MAIZE POWDER MA 1.0 IRMM CERTIFIED RE-F 5 KG YEAST EXTRACT SERVABACTERPOWDER POLYOXIN B |
Toxicity
rat and mouse acute oral LD50>20000mg/kg, rat acute percutaneous LD50>12000mg/kg, rat acute inhalation LD50>10mg/L. No irritation to rabbit eyes and skin. No carcinogenic, teratogenic and mutagenic effects were found in animal experiments. Carp LC50>40mg/L (48h), Daphnia LC50>40mg/L (3h). Low toxicity to bees, LD50>1000mg/animal.
use
Broad-spectrum antimicrobial fungicides with systemic conduction. Its mechanism of action is to interfere with the biosynthesis of chitin in cell wall. Some fungi of Ascomycetes, Basidiomycetes and Seminobacteria that use chitin as the matrix to form the cell wall, the bud tube and mycelium will locally expand, rupture, and overflow the cell contents after contacting the agent, leading to death. Mainly used to prevent and control wheat powdery mildew, ginseng black spot, tobacco red star disease, cucumber downy mildew, melon wilt, rice sheath blight, apple spot deciduous disease, strawberry and grape gray mold, tree blight and sheath disease, etc. A variety of fungal diseases. For example, the use of Baolian 10% wettable powder 1000~2000 times solution can control wheat powdery mildew and rice sheath blight, if the dosage of 15~22.5g/100m2 in the flowering period to start spraying, can control tomato, strawberry and other crops gray mold, spray once every 7 days, a total of 3~4 times. For apple spotted defoliation, spray with 50~100mg/L polyantimycin solution during the apple spring shoot growth period and the peak period of onset.
production method
1. the sand and soil pipe is connected to the inclined plane (aseptic operation) at a constant temperature of 28 ℃. when the spores grow and mature, the inclined spores are connected to the inclined plane, and the inclined plane is used for production after constant temperature cultivation. Then seed culture (seed tank), fermentation (fermentation tank), acidification, concentration, spray drying and crushing are carried out to obtain the product.
2. Process of polyantamycin biosynthesis:
sand tube strain & rarr; Inclined strain [10~14d,26 ℃]& rarr; Shake flask seeds [36~48h,28 ℃]& rarr; Seed tank seeds [24h,28 ℃]& rarr; Fermentation [90~120h,28 ℃] is put into a tank, and the fermentation liquid is acidified (pH value is 3~4)& rarr; packaging
Streptomyces golden (Streptomyces aureo chromogenes).
the formula of bevel culture bevel culture medium is as follows.
maltose 0.5%; Corn flour 0.5%; Yeast extract 0.4%; Agar 2%; The pH value is 6.3 and cultured at 26 ℃ for 10~14 days after inoculation.
Shake flask seed culture medium formula: soybean cake powder (cold pressed) 5%; Soybean oil 2%; Glucose 1%;KH2PO4 0.1%
adjust the pH value to 6.5 and add calcium carbonate to 0.3%. The tank pressure is 4.9 × 10Pa, the tank temperature is 28 ℃, the ventilation rate is 1:1, and the mechanical stirring culture is 24h. Fermentation medium formula of fermentation tank:
soybean cake powder 2%; Corn flour 1.5%; Maltose 4%; Yeast powder 0.4%; Fish meal 0.5%; Sodium chloride 0.1%; Foaming oil 0.5%
adjust the pH value to 6.5 and add calcium carbonate to 0.3%. The tank pressure is 0.05MPa, the tank temperature is 28 ℃, the ventilation rate is 1:1 (less in the front and late stages, more in the middle stage), and the fermentation time is 90~120h. Add oxalic acid after the tank, pH 3~4.
Fermentation broth concentration The fermentation broth is acidified and then concentrated under reduced pressure with a vacuum film concentrator. The feed liquid temperature is 40~60 ℃, the pressure is 1.33 × 10-4~1.47 × 10-4Pa, and the flow rate is 250 kg/h.
dry, package concentrate into drying tower spray drying. The hot air temperature in the drying tower is 150 ℃, the exhaust air temperature is 75 ℃, the negative pressure in the tower, the raw powder is crushed, and the titer is adjusted to 1.5 × 10 units (1.5%) with talcum powder.